Molecular Characterization of Phylogenetically Distinct Isolates of Grapevine fanleaf virus from Iran Based on 2AHP Gene
نویسندگان
چکیده
منابع مشابه
Characterization of the Full Length Coat Protein Gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis
The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...
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A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...
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Pakbaz S, Darvishnia M, Naghavi A (2022) Phylogenetic status of Grapevine fanleaf virus isolate of Lorestan province of Iran. Plant Pathology Science 11(1):1-12. Doi: 10.2982/PPS.11.1.1 Introduction: Grapevine fanleaf virus (GFLV) is a major grapevine infecting virus in the world. Materials and Methods: Grapes showing GFLV signs were sampled from the suburbs of the city ...
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background: avian infectious bronchitis (ib), with avianinfectious bronchitis virus (ibv) as the causing agent, is a ubiquitous endemicdisease of the chicken with devastating effects on its industry. a viralmembrane surface protein called s notonly induces neutralizing antibodies but also plays an important role in virusbinding and entry to host cells. technically, s1 protein gene sequencing a...
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ژورنال
عنوان ژورنال: Indian Journal of Virology
سال: 2012
ISSN: 0970-2822,0974-0120
DOI: 10.1007/s13337-012-0057-4